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Pre-packed columns, cartridges, and guard columns for separation of biomolecules and organic/inorganic analytes; includes normal/reversed phase HPLC, UHPLC, LC-MS, HILIC, mixed-mode, ion exchange, ligand exchange, and affinity column formats.
Suitable when limited sample is available and for polar and hydrophilic compounds that are difficult to separate. MilliporeSigma™ SeQuant™ ZIC™-HILIC (5μm, 200Å) Capillary HPLC Column, 150-1 has a high-performance, zwitterionic stationa
Separate nonpolar and moderately polar compounds with high loading capacity Hypersil PREP HS Si, fully scalable from analytical to prep for USP-L3 applications.
A typical mobile phase used with normal-phase silica columns consists of a hydrocarbon solvent such as hexane or heptane, mixed with a relatively small percentage of more polar solvent.
Available in a variety of stationary phases that function in normal and/or reverse phase. MilliporeSigma LiChroCART™ HPLC Cartridge, 75-4 comes with customized packing (please specify packing material) and is 75mmL x 4mmI.D.
Ascentis™ Express OH5 columns can be used for traditional normal phase separations using non-polar, totally organic mobile phases or for aqueous normal phase chromatography with the typical mobile phases for hydrophilic interactive liquid chromatography (HILIC) of basic, acidic, zwitterionic, or neutral compounds.
For fast, high-resolution separation and purification of proteins by ion-exchange using the quaternary amine stationary phase, use Thermo Scientific™ ProSwift™ SAX-1S LC Columns.
The sterically protected, extensively endcapped diisopropyl-cyanopropylsilane stationary phase of Ascentis™ Express ES-Cyano provides a stable, reversed-phase packing that can be used for basic, acidic, or neutral compounds.