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HPLC Columns and Cartridges

Pre-packed columns, cartridges, and guard columns for separation of biomolecules and organic/inorganic analytes; includes normal/reversed phase HPLC, UHPLC, LC-MS, HILIC, mixed-mode, ion exchange, ligand exchange, and affinity column formats.
HPLC Columns (33,744)
HPLC Guard Columns and Cartridges (4,298)
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301 – 315 of 33,003 results
301

MilliporeSigma™ Supelco™ BIOshell™ A400 Protein C18 Column, 3.4 μm Particle Size (Dia. 1-4.6 mm)

BIOshell™ 400 Å Protein C18 columns contain 3.4 μm advanced Fused-Core™ particles with 400 Å pores that are specifically engineered to provide efficient separation of larger proteins.

Particle Size 3.4 μm
Stationary Phase C18 (octadecyl)
Phase Reversed Phase
Matrix C18 (octadecyl) Phase/Spherical Silica Particle Platform
Endcapped Yes
pH 1 to 9
Material Stainless Steel
Technique Fused-Core HPLC
Surface Area ̃15 m2/g
Product Line BIOshell
303

MilliporeSigma™ Supelco™ Ascentis™ Express ES-Cyano HPLC Column, 2.7 μm Particle Size

The sterically protected, extensively endcapped diisopropyl-cyanopropylsilane stationary phase of Ascentis™ Express ES-Cyano provides a stable, reversed-phase packing that can be used for basic, acidic, or neutral compounds

Pore Size 90 Å
Particle Size 2.7 μm
Stationary Phase Diisopropyl-Cyanopropylsilane
Phase Reversed Phase
Matrix Diisopropyl-Cyanopropylsilane Phase/Fused-Core Particle Platform
Endcapped Yes
Technique Fused-Core HPLC
Product Line Ascentis
304

Thermo Scientific™ Acclaim™ Trinity P2 HPLC Columns
GREENER_CHOICE

Use these mixed-mode columns for separation of multivalent cations or anions and pharmaceuticals.

Pore Size 300 Å
Solvents Compatible with most common HPLC organic solvents except for alcohols
Stationary Phase Spherical, Nanopolymer Hybrid Silica
Temperature 45°C
Phase HILIC and Ion Exchange
Endcapped Proprietary
Column Type Mixed Mode
Green Features FSC
Surface Area 100 m²/g
Max. Pressure 6000 psi (415 bar)
Particle Size 3 μm
Mobile Phase Compatibility 0 to 100% aqueous mobile phase
pH 2.5 to 7
Particle Shape Spherical silica
Product Line Acclaim
305

MilliporeSigma Ascentis™ Express 120 Å C18 pH+ (2.7 μm) HPLC Column

The HPLC Column contains 1 Pk.

Pore Size 120 Å
Particle Size 2.7 μm
Phase Reversed Phase
Product Type HPLC Column
Diameter (Metric) 4.6 mm
Material Stainless Steel
Technique HPLC
306

MilliporeSigma™ Supelco™ Ascentis™ Express 90 Å PFAS, 2.7 μm HPLC Column

The densely bonded, extensively endcapped ODS stationary phase of Ascentis™ Express PFAS provides an application tested solution for PFAS analysis.

Pore Size 90 Å
Particle Size 2.7 μm
Temperature <60°C
Matrix Fused-Core™ Silica Particle Platform Particle Platform Superficially Porous Particle
Column Type Reversed Phase
pH 2 to 9
Quantity 1 Ea.
Material Stainless Steel
Surface Area 135 m2/g
Max. Pressure 600 bar
307

MilliporeSigma™ LiChroCART™ Columns/Purospher™ STAR RP-8 Endcapped Sorbents HPLC Cartridges, 5μm

Optimized for more polar compounds

Pore Size 120 Å
Stationary Phase C8
Phase Reversed Phase
Endcapped Yes
Column Type Reversed Phase
Quantity 1/Pk.
Surface Area 330 m²/g
Max. Pressure 2175 psi (150 bar) to 2900 psi (200 bar)
Column Format Cartridge
Particle Size 5 μm
Carbon Load 17%
Product Type Cartridge
Packing Material Silica
pH 1.5 to 10.5
USP Type L7
308

TSKgel™ G4000SW HPLC Column, 17 μm Particle Size, Sold by MilliporeSigma™ Supelco™

TSKgel™ G4000SW SEC columns provide excellent separation of proteins and other biomolecules.

Pore Size 450 Å
Particle Size 17 μm
Solvents 0 to 100% aqueous soluble organic solvents
Flow Rate 8 mL/min.
Stationary Phase Diol
Temperature 10°C to 30°C
Phase Size Exclusion
Matrix Silica Particle Platform
Diameter (Metric) 21.5 mm
pH 2.5 - 7.5
Material Stainless Steel Hardware
Product Line TSKgel
USP Type L20, L33, L59
309

TSKgel™ G2000SW HPLC Column, 10 μm Particle Size, Sold by MilliporeSigma™ Supelco™

TSKgel™ G2000SW SEC columns provide excellent separation of peptides and proteins with molecular weights up to 100,000 Da.

Pore Size 125 Å
Particle Size 10 μm
Solvents 0 to 100% aqueous soluble organic solvents
Flow Rate 1.2 mL/min.
Stationary Phase Diol
Temperature 10°C to 30°C
Phase Size Exclusion
Matrix Silica Particle Platform
Diameter (Metric) 7.5 mm
pH 2.5 - 7.5
Product Line TSKgel
USP Type L20, L59
310

TSKgel™ ODS-120A HPLC Column, 10 μm Particle Size, Sold by MilliporeSigma™ Supelco™

TSKgel™ ODS-120A columns contain 150 Å pore size silica particles. The exclusion limit of 10,000 Da makes TSKgel™ ODS-120A columns a good choice for the reversed phase chromatography of peptides, small proteins, and environmental samples such as poly-aromatic hydrocarbons.

Pore Size 150 Å
Particle Size 10 μm
Solvents 0 to 100% organic solvent
Carbon Load 20%
Stationary Phase C18 (octadecyl)
Temperature 0°C to 45°C
Phase Reversed Phase
Matrix Spherical Silica
Length (Metric) 30 cm
Endcapped No
pH 2.0 - 7.5
Product Line TSKgel
USP Type L1
311

TSKgel™ UP-SW Guard Column, 2 μm Particle Size, Sold by MilliporeSigma™ Supelco™

For use with TSKgel™ UP-SW3000 HPLC Columns

Pore Size 250 Å
Solvents 0-30% Aqueous Soluble Organic Solvents
Stationary Phase Diol
Description Salt Concentration: ≤ 0.5 M
Length (Metric) 2 cm
Diameter (Metric) Inner 4.6 mm
Temperature (Metric) Maximum 30°C
Particle Size 2 μm
Packing Material Silica Particle Platform
For Use With (Equipment) TSKgel™ UP-SW3000 HPLC Columns (80023448, 800234489)
pH 2.5 to 7.5
Technique Size Exclusion (SEC)
Product Line TSKgel
USP Type L20, L59
312

TSKgel™ G2000SW HPLC Column, 13 μm Particle Size, Sold by MilliporeSigma™ Supelco™

TSKgel™ G2000SW SEC columns provide excellent separation of peptides and proteins with molecular weights up to 100,000 Da.

Pore Size 125 Å
Particle Size 13 μm
Solvents 0 to 100% aqueous soluble organic solvents
Flow Rate 8 mL/min.
Stationary Phase Diol
Temperature 10°C to 30°C
Phase Size Exclusion
Matrix Silica Particle Platform
Diameter (Metric) 21.5 mm
pH 2.5 - 7.5
Material Stainless Steel Hardware
Product Line TSKgel
USP Type L20, L59
313

TSKgel™ G3000SW HPLC Column, 10 μm Particle Size, Sold by MilliporeSigma™ Supelco™

TSKgel™ G3000SW SEC columns provide excellent separation of proteins and other biomolecules with molecular weights up to 500,000 Da.

Pore Size 250 Å
Particle Size 10 μm
Solvents 0 to 100% aqueous soluble organic solvents
Flow Rate 1.2 mL/min.
Stationary Phase Diol
Temperature 10°C to 30°C
Phase Size Exclusion
Matrix Silica Particle Platform
Diameter (Metric) 7.5 mm
pH 2.5 - 7.5
Product Line TSKgel
USP Type L20, L59
314

TSKgel™ G2000PW HPLC Column, 12 μm Particle Size, Sold by MilliporeSigma™ Supelco™

Use the TSKgel™ G2000PW HPLC column for analysis of water-soluble polymers with molecular weights up to 2,000 Da.

Pore Size 125 Å
Particle Size 12 μm
Solvents ≤20% organic solvent
Flow Rate 1.2 mL/min.
Stationary Phase Hydroxylated Methacrylate
Temperature 80°C
Phase Size Exclusion
Matrix Polymer Particle Platform
Diameter (Metric) 7.5 mm
pH 2.0 - 12
Product Line TSKgel
USP Type L25, L38, L39
315

TSKgel™ G3000PW HPLC Column, 12 μm Particle Size, Sold by MilliporeSigma™ Supelco™

TSKgel™ G3000PW SEC columns can be used for the analysis of water-soluble polymers of less than 50,000 Da molecular weight.

Pore Size 200 Å
Particle Size 12 μm
Solvents ≤20% organic solvent
Flow Rate 1.2 mL/min.
Stationary Phase Hydroxylated Methacrylate
Temperature 80°C
Phase Size Exclusion
Matrix Polymer Particle Platform
Diameter (Metric) 7.5 mm
pH 2.0 - 12
Product Line TSKgel
USP Type L37, L38, L39